Primer

Part:BBa_K5406004:Design

Designed by: Efstathions Alonaris, Anna Nikoleta Griva, Vassili N. Kouvelis, Kristian Verdhi   Group: iGEM24_Athens   (2024-09-21)


Forward Primer for GshFss Sequencing


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For a set of compatible primers to be designed, the sequence of the 5' end restriction enzyme had to be used as well. In our case, that was the BamHI for the forward primer. In addition, the help of professor Vassili N. Kouvelis needs to be aknowleged

Source

This part was made partially using Primer BLAST, a tool of the National Center for Biotechnology Information (NCBI) of the United States of America. The sequence for this part was made using as a template the GShFss gene as figured in part BBa_K5406000.

References